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saccharomyces cerevisiae leishmania clinical isolate activating factor sterol biosynthesis characterization vitro squalene synthase calpain inhibitor linked extracellular proteinase yeast platelet activating protease alkaloid plasma membrane interaction cancer phytomonas serpens peptidase ultrastructure herpetomonas samuelpessoai multidrug resistance fluconazole trypanosoma novel candida albicans trypanosomatid kinase